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A series of preliminary reports and studies of different aspects of the discoveries have already been published, while a comprehensive illustrated overview was published in The discussion of a high or low chronology in this region has recently had particular prominence [10][11]. Surprisingly little work has been conducted on bone collagen preservation in the tropics, although it is well known that collagen survival is subject to a number of factors that work synergistically to facilitate degradation; namely time, temperature, bacterial presence, geochemistry and hydrology [ 3233 ]. Dates based on 14 C dating methods have had wide error margins and the complexities of the calibration curve for the final centuries of the second millennium BC preclude the precise dating of a single sample using 14 C techniques alone. Two are included where we need to account for the hiatus between Phases 1. These two samples were selected due to the quality of their collagen fingerprints, larger size and deposition location deep within Green Cave Chamber 5; see Table 2. Collagen peptide fingerprints were obtained following methods modified from Buckley et al. ZooMS can reduce time and expense, whilst lowering the risk of unreliable date attainment, preventing unnecessary sample destruction and providing additional information on species identification.

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  • Dating the End of the Greek Bronze Age A Robust RadiocarbonBased Chronology from Assiros Toumba
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    Collagen peptide fingerprints were obtained following methods modified from Buckley et al. Table S7.

    Dating the End of the Greek Bronze Age A Robust RadiocarbonBased Chronology from Assiros Toumba

    Deger-Jalkotzy S. View Article Google Scholar 5. Similarly, few sites have provided more than a handful of charcoal samples, usually far from ideal for dating purposes.

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    However, this technique is both time consuming and involves destruction of samples to obtain thin sections for light microscopy. Andrew T.

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    It is shown in Fig. These are not shown on the figure. Data Availability: The authors confirm that all data underlying the findings are fully available without restriction.

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    These are not shown on the figure.

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    Within the settlement, it was possible to distinguish between interiors which were kept more or less clean, unroofed yards where rubbish was allowed to accumulate and streets where gravel and broken pottery was regularly strewn in order to maintain a firm surface in all weathers.

    Within all the Cayman Brac sub-fossil samples that were successfully dated, these parameters fell within the optimum limits for reliable dating [ 3 ].

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    Egerton1, 2 Andrew T. Oak timbers from Assiros used for dendrochronology.

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    Estimating the chance of success of archaeometric analyses of bone: UV-induced bone fluorescence compared to histological screening. Assiros Toumba is a settlement mound situated at the NE end of the Langadas Basin, some 25 km inland from modern Thessaloniki.

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    Bones deposited in caves may also become encased in calcareous matrices, such as is common on the Cayman Islands Fig 1increasing the likelihood of exceptional protein preservation.

    Most cattle Bos from Phase 5 and later ate an atypical diet with high levels of C-4 plants.

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    In particular, the 36 domestic animal bone samples, formed a consistent sequence with only two outliers [23] Table S6 in File S1. The only clear hiatus occurred between the end of Phase 1. In consequence, the absolute dates from the Assiros contexts can be transferred to equivalent contexts in southern Greece, and used to re-date the successive pottery phases there.

    DOCX Click here for additional data file.

    Author: Yozragore

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    1. Radiocarbon AMS dates from Assiros obtained from bones with associated analytical and context data. Assiros Phase 9 with granaries.

    2. However, one of the most appropriate means to achieve such a framework—radiocarbon dating—is significantly costly, both in terms of equipment and time [ 4 ].

    3. Collagen fingerprinting of archaeological bone and teeth remains from Domuztepe, South Eastern Turkey. Palaeogeography, Palaeoclimatology, Palaeoecology.

    4. An uninterrupted stratigraphic sequence of building levels covers more than years, while the preservation of substantial charred structural timbers from four phases has enabled precise dates to be established for the cutting of these timbers, using the technique of dendrochronological wiggle-matching DWM. Discussion Collagen survival in the tropics Surprisingly little work has been conducted on bone collagen preservation in the tropics, although it is well known that collagen survival is subject to a number of factors that work synergistically to facilitate degradation; namely time, temperature, bacterial presence, geochemistry and hydrology [ 3233 ].