We are also grateful to Staffan Arvidson for valuable comments on the manuscript. Advance article alerts. Identification and characterization of SarH1, a new global regulator of virulence gene expression in Staphylococcus aureus. Degradation of elastin by a cysteine proteinase from Staphylococcus aureus. To test whether the positive effect of sarR on aur and sspA transcription really is mediated via sarAthe effect of inactivating the sarR locus in a sarA gene background was studied by measuring aur and sspA mRNA levels in isogenic sarA single PC and sarA sarR double WA mutants. Occurrence of an extracellular serineproteinase among Staphylococcus aureus strains. This suggests that repression of aur by sarA is sarR -dependent.
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We are also grateful to Staffan Arvidson for valuable comments on the manuscript.
RCSB PDB 1BQB AUREOLYSIN, STAPHYLOCOCCUS AUREUS METALLOPROTEINASE
The bacterial strains and plasmids used in this study are listed in Table 1. Receive exclusive offers and updates from Oxford Academic.
However, we found that sarR acted as a direct stimulator, i. It should be pointed out that all strains tested in this study showed similar growth in in vitro batch cultures, i.
from S. epidermidis (17) and the aureolysin gene cloned in this work. Staphylococcus aureus expresses several proteases, which are of S. aureus strains was used to determine the range of aureolysin (aur) gene polymorphism.
codon of the aur gene and represent positions of recombination breakpoints. . Department of Medical Microbiology, University Medical Center. Formerly, S. aureus infections were confined to healthcare settings, afflicting the. Next, a USA LAC aureolysin mutant was generated using a.
As it forms the basis of the work described herein, we first set out to confirm.
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We could therefore conclude that sarR stimulated aur and sspA transcription independent of sarA.
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Regulation and characterization of rot transcription in Staphylococcus aureus. Sites of cleavage and generation of chemotactic activity. To test whether the positive effect of sarR on aur and sspA transcription really is mediated via sarAthe effect of inactivating the sarR locus in a sarA gene background was studied by measuring aur and sspA mRNA levels in isogenic sarA single PC and sarA sarR double WA mutants.
Indeed, inactivation of sarR in the sarA mutant resulted in a three- to ninefold decrease of aur transcription and a two- to ninefold decrease in sspA transcription Fig 2bwhich is consistent with a slightly reduced zone of proteolysis on casein agar Fig.
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|Novick Decreased amounts of cell wall-associated protein A and fibronectin-binding proteins in Staphylococcus aureussarA mutants due to up-regulation of extracellular proteases.
Oscarsson et al. Thus, our present data support a model in which sarR positively influences aur and sspA in a direct way, consistent with DNA-binding experiments Tegmark et al.
This is consistent with the less dense appearance of the zone of proteolysis of WA relative to on casein agar Fig. Effect of inactivation of sarR in a wild-type, sarA and agr rot mutant background on aur and sspA transcription. Regulation and characterization of rot transcription in Staphylococcus aureus.
aureus secreted protease aureolysin in processing PSMs is This work demonstrates that IMS can serve as a useful tool to go NIH HHS/United States; Howard Hughes Medical Institute/United States.
PDF | Staphylococcus aureus expresses several proteases, which are thought to contribute to the virulence of this bacterium.
Here we focus on aureolysin, the. In keeping with LL degradation by aureolysin, S. aureus strains that. Emory University School of Medicine, Atlanta, Laboratories of Microbial Pathogenesis, This work was supported by grants 3 B 22 and 3 P05A
Cleavage of human immunoglobulins by serine proteinase from Staphylococcus aureus.
Proteolytic activity of Staphylococcus aureus strains isolated from the colonized skin of patients with acute-phase atopic dermatitis.
Population structure and toxin gene profiles of Bacillus cereus sensu lato isolated from flour products. Furthermore, the indicated stimulatory effect of sarR on rot transcription Fig. Oscarsson, unpublished results. However, as judged by the size of proteolytic zones on casein agar, inactivation of sarR had no apparent effect on protease production Oscarsson et al.
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|However, we found that sarR acted as a direct stimulator, i.
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Regulatory pathways by which sarR positively influences transcription of aur and sspA during the postexponential phase of growth, when they are maximally expressed, are depicted in Fig.
Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. This prompted us to re-evaluate previous results and to further analyze the role of sarR in aur and sspA transcription more thoroughly. Consistent with this, WA, relative to WA, had a smaller proteolytic zone on casein agar that was less dense Fig.
In vitro studies have demonstrated that staphylococcal proteases can cleave and degrade a number of important host proteins, including the heavy chains of all human immunoglobulin classes, plasma proteinase inhibitor and elastin Potempa et al. Download all figures.