I think I might try lowering the cpbs, even though 2 is the lowest Roche proposes. Add the reagents in the order they are listed in the tables. Originally Posted by Christina Two titration options are available. Cancel Delete. Change language. Have you tried repeating the reactions with lower cpb values? If this happens, either repeat the quantitation fluorometric assay and performthe titration with the corrected results, or simply repeat the emulsion titration with more orless input DNA. Variants of this procedure exist to accommodate different types ofDNA lib raries and different number of beads requirements for the experiment.
emPCR Amplification Method Manual Lib-L SV Refer to this manual for the.
Video: Empcr method manual lib a svee Assay Tricks
a syringe and blunt needle, as described for the SVE procedure. made of 2 pooled emulsions, referred to as 2×SVE and destined to be loaded in M/S.
described in the GS FLX Titanium emPCR Method Manual Lib-L SV, (the. Titanium General Library Preparation Method Manual, April ). DNA Library in the GS FLX Titanium emPCR Method Manual Lib-L SV. a syringe and blunt needle, as described for the SVE procedure, in the GS FLX Titanium emPCR.
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Remove anddiscard the supernatant. The lower DNA input reactions e.
Bead enrichment ( FLX Titanium) Sample Prep / Library Generation. Platform (so I am following the emPCR Method Manual – Lib-A SV) and I instead of the proposed by the protocol ( × 10^6 for 1×Amp SVE) in. Caution: The improved GS Titanium emPCR Reagents SV (Lib-L), mat.
Calculation of Bead enrichment ( FLX Titanium) SEQanswers
no. Place 2 ml (or 1 ml for SVE) of 5X Mock Amplification Mix in a 15 ml Falcon tube. b. of the GS FLX Titanium emPCR Method Manual or step 6.
Certainly they did not seem broken.
Main languages. Hi Christina, What cpb values did you use for your inputs? Table 2 shows the settings of the CASY models for bead counting. Remember Me?
GS FLX Titanium emPCR MM liba small volume HIGH
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